Annexe I: Publications

نویسندگان

  • Cristian Ruiz
  • Ana Blanco
  • Javier Pastor
  • Pilar Diaz
چکیده

The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C4^C8) substrates, and poor activity on C18 substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases. 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

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تاریخ انتشار 2006